March 2017

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Long-term Results of Living Donors in Simultaneous Kidney and Liver Transplantations.

Posted by on 28 Mar 2017 | Tagged as: Hepatitis B Alternative Medicine

Related Articles Long-term Results of Living Donors in Simultaneous Kidney and Liver Transplantations. Transplant Proc. 2017 Apr;49(3):403-406 Authors: Unek T, Egeli T, Özbilgin M, Çelik A, Atilla K, Ağalar C, Arslan NÇ, Karademir S, Bora S, Gülay H, Derici ZS, Astarcıoğlu I Abstract INTRODUCTION: Because of the shortage of organs available for transplantation, living related sequential transplantation with the use of liver and a kidney from the same donor has emerged as a reasonable therapeutic alternative. However, there is insufficient literature about the complications that living donors experience after simultaneous kidney and liver transplantations. METHODS: From December 2001 to October 2009, 5 living donors provided simultaneous donation of livers and kidneys and 1 living donor donated first her kidney and then her liver. Demographic data of the donors and information concerning the surgery and postoperative observation were collected prospectively. RESULTS: All of the donors were female. The median age was 27.5 (range, 19-36) years. Indications requiring the simultaneous transplantation of livers and kidneys were primary hyperoxaluria type 1 (PH1) in 5 potential recipients and cirrhosis due to chronic hepatitis B infection and idiopathic chronic renal insufficiency in 1 potential recipient. Four recipients underwent right hepatectomy (segments 5-8) and right nephrectomy; 1 recipient underwent left hepatectomy (segments 2-4) and right nephrectomy; and 1 recipient underwent left lobectomy (segments 2-3) and right nephrectomy. There were no complications except in 1 donor (postoperative ileus). No donor developed hypertension or microalbuminuria. CONCLUSIONS: With the right indications, appropriate preoperative evaluation, meticulous surgical technique, proper postoperative care, and long-term close monitoring to minimize morbidity and mortality risks, liver and kidney donation from the same donor can be considered for simultaneous kidney and liver transplantation. PMID: 28340800 [PubMed – in process]

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Long-term Results of Living Donors in Simultaneous Kidney and Liver Transplantations.

Differential regulation of hepatitis B virus core protein expression and genome replication by a small upstream open reading frame and naturally…

Posted by on 07 Mar 2017 | Tagged as: Hepatitis B Alternative Medicine

Related Articles Differential regulation of hepatitis B virus core protein expression and genome replication by a small upstream open reading frame and naturally occurring mutations in the precore region. Virology. 2017 Mar 02;505:155-161 Authors: Zong L, Qin Y, Jia H, Ye L, Wang Y, Zhang J, Wands JR, Tong S, Li J Abstract Hepatitis B virus (HBV) transcribes two subsets of 3.5-kb RNAs: precore RNA for hepatitis B e antigen (HBeAg) expression, and pregenomic RNA for core and P protein translation as well as genome replication. HBeAg expression could be prevented by mutations in the precore region, while an upstream open reading frame (uORF) has been proposed as a negative regulator of core protein translation. We employed replication competent HBV DNA constructs and transient transfection experiments in Huh7 cells to verify the uORF effect and to explore the alternative function of precore RNA. Optimized Kozak sequence for the uORF or extra ATG codons as present in some HBV genotypes reduced core protein expression. G1896A nonsense mutation promoted more efficient core protein expression than mutated precore ATG, while a +1 frameshift mutation was ineffective. In conclusion, various HBeAg-negative precore mutations and mutations affecting uORF differentially regulate core protein expression and genome replication. PMID: 28260621 [PubMed – as supplied by publisher]

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Differential regulation of hepatitis B virus core protein expression and genome replication by a small upstream open reading frame and naturally…